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Tryptone 12 Yeast extract 24 (NH4)2SO4 3
5 Soluble starch 7
7 NH4Cl 26
discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107
Yeast Nitrogen base without Amino acids and without Calcium Pantothenate Size:1000g Tryptone 12 Yeast extract 24Calcium pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium pantothenate or to replace this vitamin. Suspend 6. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature
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