Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g Pre-Sporulation Medium is used for

Pre-Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly

2 at 25°C Suspend 18 gram powdered medium in 1 litre distilled water

As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids

In similarity with plant Agar

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g Pre-Sporulation Medium is used forCalcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room