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minus Arginine and without Cysteine
Ascorbic acid and Magnesium sulphate are added to stimulate growth
3g) in one litre is 10X
In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells
Super Broth Size:250g minus Arginine and without CysteineSuper broth is an extremely rich medium with highly elevated levels of Tryptone and Yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained. Formula g l Tryptone 35 Yeast Extract 20 NaCl 5 Final pH 7. 0 0. 2 at 25C Suspend 60 gram powdered medium in 1 litre distilled water Store dry at room temperature.
