Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g FeCl3 are omitted from this

FeCl3 are omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper and iron or to replace these elements

Solubility Freely soluble in water Specific Optical Rotation 104 ~ 121 Water <6% Absorbance 570 nm <0

Adjust to pH 6

5 Thin Layer Chromatogram One Spot Potency >720 µ/mg Store at 2 - 8°C

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g FeCl3 are omitted from thisThiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room